Completed chloroplast genome of Zoysia matrella (L.) Merr. isolated in Korea is 135,888 bp long (GC ratio is 38.4\%) and has four subregions: 81,370 bp of large single copy (36.3\%) and 12,594 bp of small single copy (32.7\%) regions are separated by 20,962 bp of inverted repeat (44.1\%) regions including 130 genes (83 protein-coding genes, eight rRNAs, 38 tRNAs, and one pseudogene). 28 SNPs and 57 INDELs were identified ss intraspecific variations against previously sequenced chloroplast genome. Phylogenetic trees show that Z. matrella and Z. tenuifolia (=Z. pacifica) are clustered in one clade with low level of variations on chloroplast genomes.